"Life Time Rights: My mom died recently. Years ago there was some property that was given to my mom, and then with love she gave it to my brother. He gave my mom life time rights on the land. However, my brother died before my mom and left a wife and kids. Does my mom's will override the deed."
However, if the deed your mother gave to your brother that delineated her life estate had a contingency that the ownership of the property would only be transferred to your brother if he survived her, then your brother's interests (called a contingent remainder interest) would lapse on his death before your mother. If this were the scenario, then your mother would own the entire interest in the property and on her death the property would be subject to her will.
So, just like the answer to every legal question, it depends! You should have a real estate attorney in the area where your mother's property is located review the title report and deeds and help you determine precisely what the legal rights are as they will affect the ownership of the particular property in a dramatic manner.
Another point, let's say that the result of the deeds is not what the family intended. If that is the case and everyone is willing to cooperate, it might be possible for certain people (e.g., your brother's wife) to disclaim part of their interests in the property and shift the ownership. This will be very tricky based on the above facts, especially if your brother's children are minors. However, a creative probate attorney might be able to help. Another approach to correct the situation if the ownership is not what the family intended and if (which might be a big if) is for those receiving the property inappropriately (e.g. your brother's wife) to gift interests in the property to those who should have an interest. You need to have the lawyer helping you address the annual gift tax exclusion.
You need to be careful with property handling this because you don't want to create any issues with respect to the title to the property that might make it more difficult to sell later.
Finally, there are some income tax issues to consider. The property should get a step up in tax basis on your mother's death if she in fact held a life estate. That might eliminate any capital gains tax if the property is sold. Therefore, the resolution of the exact nature of the legal ownership should then be reviewed with your accountant to be sure you get the best tax result.